Motility defects in Campylobacter jejuni defined gene deletion mutants caused by second-site mutations

Stefan De, Srishti Gupta, Abiyad Baig, Joanna L'Heureux, Elsa Pont, Dominika P Wolanska, Duncan Maskell & Andrew Grant
Genetic variation due to mutation and phase-variation has a considerable impact on the commensal and pathogenic behaviours of Campylobacter jejuni. In this study, we provide an example of how second-site mutations can interfere with gene function analysis in C. jejuni. Deletion of the flagellin B gene (flaB) in C. jejuni M1 resulted in mutant clones with inconsistent motility phenotypes. From the flaB mutant clones picked for further analysis, two were motile, one showed intermediate motility,...

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