The essential genome of Streptococcus agalactiae.

Thomas A Hooven, Andrew J Catomeris, Leor H Akabas, Tara M Randis, Duncan Maskell, Sarah E Peters, Sandra Ott, Ivette Santana-Cruz, Luke J Tallon, Hervé Tettelin & Adam J Ratner
BACKGROUND: Next-generation sequencing of transposon-genome junctions from a saturated bacterial mutant library (Tn-seq) is a powerful tool that permits genome-wide determination of the contribution of genes to fitness of the organism under a wide range of experimental conditions. We report development, testing, and results from a Tn-seq system for use in Streptococcus agalactiae (group B Streptococcus; GBS), an important cause of neonatal sepsis. METHODS: Our method uses a Himar1 mini-transposon that inserts at genomic TA...
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