Defining the ABC of gene essentiality in streptococci

Amelia RL Charbonneau, Oliver P Forman, Amy K Cain, Graham Newland, Carl Robinson, Mike Boursnell, Julian Parkhill, James A Leigh, Duncan Maskell & Andrew S Waller
Abstract Background Utilising next generation sequencing to interrogate saturated bacterial mutant libraries provides unprecedented information for the assignment of genome-wide gene essentiality. Exposure of saturated mutant libraries to specific conditions and subsequent sequencing can be exploited to uncover gene essentiality relevant to the condition. Here we present a barcoded transposon directed insertion-site sequencing (TraDIS) system to define an essential gene list for Streptococcus equi subsp. equi, the causative agent of strangles in horses, for the...

This data repository is not currently reporting usage information. For information on how your repository can submit usage information, please see our documentation.