Interpreting ELISA analyses from wild animal samples: Some recurrent issues and solutions

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1. Many studies in disease and immunological ecology rely on the use of assays that quantify the amount of specific antibodies (immunoglobulin) in samples. Enzyme-Linked Immuno Sorbent Assays (ELISAs) are increasingly used in ecology due to their availability for a broad array of antigens and the limited amount of sampling material they require. Two recurrent methodological issues are nevertheless faced by researchers: (i) the limited availability of immunological assays and reagents developed for non-model species,...

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