¹³C magnetic resonance spectroscopy measurements with hyperpolarized [1-¹³C] pyruvate can be used to detect the expression of transgenic pyruvate decarboxylase activity in vivo

Piotr Dzien, Sui-Seng Tee, Mikko I Kettunen, Scott K Lyons, Timothy J Larkin, Kerstin N Timm, De-En Hu, Alan Wright, Tiago B Rodrigues, Eva M Serrao, Irene Marco-Rius, Elizabeth Mannion, Paula D‘Santos, Brett W Kennedy & Kevin Brindle
Purpose Dissolution dynamic nuclear polarization can increase the sensitivity of the 13C magnetic resonance spectroscopy experiment by at least four orders of magnitude and offers a novel approach to the development of MRI gene reporters based on enzymes that metabolize 13C-labeled tracers. We describe here a gene reporter based on the enzyme Pyruvate Decarboxylase (EC 4.1.1.1), which catalyzes the decarboxylation of pyruvate to produce acetaldehyde and carbon dioxide. Methods Pyruvate Decarboxylase from Zymomonas mobilis (zmPDC),...

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