Transcriptome Analysis Reveals Extensive Alternative Splicing-Coupled Nonsense-Mediated mRNA Decay in a Human Cell Line

Courtney French, Gang Wei & Steven Brenner
To further explore the regulatory potential of nonsense-mediated mRNA decay (NMD) in human cells, we globally surveyed the transcripts targeted by this pathway via RNA-Seq analysis of HeLa cells in which NMD had been inhibited. We first identified those transcripts with both a premature termination codon more than 50 nucleotides upstream of an exon-exon junction (50nt rule) and a significant increase in abundance upon NMD inhibition. Remarkably, at least 2,793 transcripts derived from 2,116 genes...
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