We used Translating Ribosome Affinity Purification coupled with next generation sequencing (TRAP-seq) to profile mouse tubular epithelial cells during the course of injury and repair using the Kim-GCE mouse model. This novel mouse model allows lineage tracing of injured mouse tubular epithelial cells, which upregulate Kim1, and translational profiling by ribosomal pull down when crossed to the EGFP-L10a mouse. Transcriptional profiling was done at day 2, 7, and 14 after bilateral ischemia reperfusion injury.
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