Assessing the reliability of gene expression measurements in very-low-numbers of human monocyte-derived macrophages

Carsten Geiß, Gregorio Alanis-Lobato, Miguel Andrade & Anne Régnier-Vigouroux
Tumor-derived primary cells are essential for in vitro and in vivo studies of tumor biology. The scarcity of this cellular material limits the feasibility of experiments or analyses and hence hinders basic and clinical research progress. We set out to determine the minimum number of cells that can be analyzed with standard laboratory equipment and that leads to reliable results, unbiased by cell number. A proof-of-principle study was conducted with primary human monocyte-derived macrophages, seeded...
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