Heterologe Expression und physikochemische Charakterisierung der Polyphenoloxidasen aus dem Cabernet Sauvignon (Vitis vinifera)

Even Solem
Type-3 copper proteins are ubiquitously distributed. Polyphenol oxidases (PPOs), which are characteristic for green plants, are classified as enzymatic tyrosinases and catechol oxidases. In the present work, three PPO sequences from the grape Vitis vinifera L. cv. Cabernet Sauvignon (L-VvPPOcs-1, L-VvPPOcs-2 and L-VvPPOcs-3) were obtained from cDNA by cloning and sequencing. These were defined as two different isoforms, one of which is presumably represented by two alleles (L-VvPPOcs-1 and L-VvPPOcs-2). Here, L-VvPPOcs-1 had a sequence...
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