A critical assessment for the value of markers to gate-out undesired events in hla-peptide multimer staining protocols

Sebastian Attig, Leah Price, Sylvia Janetzki, Michael Kalos, Michael Pride, Lisa McNeil, Tim Clay, Jianda Yuan, Kunle Odunsi, Axel Hoos, Pedro Romero & Cedrik M. Britten
Background: The introduction of antibody markers to identify undesired cell populations in flow-cytometry based assays, so called DUMP channel markers, has become a practice in an increasing number of labs performing HLA-peptide multimer assays. However, the impact of the introduction of a DUMP channel in multimer assays has so far not been systematically investigated across a broad variety of protocols. Methods: The Cancer Research Institute%27s Cancer Immunotherapy Consortium (CRI-CIC) conducted a multimer proficiency panel with...
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