Two dimensional infrared four wave mixing spectroscopy of simple molecules, peptides and proteins

Paul Murray Donaldson
In this thesis, measurements of electron-vibration-vibration two dimensional infrared (EVV 2DIR) spectra are demonstrated for the first time from aliphatic, aromatic and amide containing compounds. Bioanalytical applications of EVV 2DIR spectroscopy are explored and the first EVV 2DIR measurements on proteins reported. Operational protocols for collecting EVV 2DIR spectra are described and it is shown for the case of benzene Fermi resonances how excitation pulse ordering can be used to isolate different EVV coherence pathways,...
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