COUPLING DNA LABELING AND NEXT-GENERATION SEQUENCING TECHNIQUES TO CHARACTERIZE METABOLICALLY-ACTIVE BACTERIA IN NONTRADITIONAL IRRIGATION WATER

Leena Malayil
Bacteria are ubiquitous in irrigation water resources and can include pathogens that may compromise food safety and public health. However, only a small fraction of total bacterial community members in water can be identified through standard culture-based laboratory methods. 16S rRNA and shotgun sequencing techniques have improved the identification of non-culturable bacteria in water resources. Nevertheless, because sequencing approaches are nucleic-acid based, they are unable to differentiate between the proportion of bacterial communities identified that...
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