Whole-genome transcriptome profiling of E12.5 Wnt1-Cre;Tgfbr2flfl by microarray of maxilla and mandible

Paul Thomas, Pedro Sanchez & Yang Chai
We performed the global gene expression profiling analysis of the proximal (P ) and distal (D) regions of the mandible and proximal (P ) and distal (D) regions of the maxilla using microarray. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the GeneChip® IVT Labeling Kit and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cRNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe...
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