Single cell RNA-sequencing of E11.5 Crect; ROSA26-lacZ mouse fusing upper lip and primary palate

Joan Hooper, Kenneth Jones & Trevor Williams
Only mouse embryos that were positive for both Crect, an ectoderm specific Cre recombinase transgene, and the ROSA26 LacZ reporter allele were used for this study. Not that these mice are on a mixed genetic background. After isolation of E11.5 embryos with 45 somites, the face was isolated from the head using published procedures. Subsequently, the region of the upper face where the nasal and maxillary prominences are juxtaposed was microdissected out using forceps so...
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