Gene expression profiling of the palate in compound mutant mice with a conditional inactivation of Tgfbr2 and conditional haploinsufficiency of Alk5 at embryonic day E14.5

Pedro Sanchez, Richard Pelikan & Yang Chai
We performed global gene expression analyses of the palate in Wnt1-Cre;Tgfbr2^fl/fl ;Alk5^fl/+ compound mutant mice at embryonic day E14.5. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the Enzo BioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39000 transcripts. Microarrays were hybridized, processed...
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