Gene expression profiling of the tongue primordium in mice with a conditional inactivation of Smad4 at embryonic day E13.5

Pedro Sanchez, Richard Pelikan & Yang Chai
We performed global gene expression analyses of the tongue primordium in Myf5-Cre;Smad4^fl/fl and Smad4^fl/fl control mice at embryonic day E13.5. Total RNA samples (1 ug per sample) were converted into biotin-labeled cRNA using the Enzo BioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39000 transcripts. Microarrays were hybridized,...
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