What miRNAs are expressed in the E13.5 mouse midfacial tissue?

Annette Werner, David Clouthier, John Postlethwait, Kristin Artinger & Peter Batzel
Researching what miRNAs are expressed in the E13.5 mouse midfacial tissue. Methods: 5 dissected maxillas (1.2 mg w/w per maxilla; 3.2 ug of purified miRNA); 3 dissected FNP (4.5 mg w/w per FNP; 4.8 ug of purified miRNA); 9 dissected palatal shelves (0.8 mg w/w per palatal shelves; 2.6 ug of purified miRNA); miRNA extracted using the Norgen miRNA Purification Kit, miRNA libraries prepared by ligation of barcoded adapters on 5' ends of small-RNA sequences,...
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