Skull vault formation in individual Chi-/+ fish

Matthew Harris & Shannon Fisher
An individual fish was imaged successively from 14-36 dpf by confocal microscopy, during the period of induction and growth of the frontal and parietal bones. The fish was transgenic for col1a1a:gfp, marking chondrocytes, and for sp7:mcherry, marking osteoblasts. The fish was a heterozygous mutant for Chihuahua, and mutation in the col1a1a gene that models human Osteogenesis Imperfecta.
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