Gene expression profiling of palatal and mandibular tissue in Msx1-/- mice

Pedro Sanchez, Richard Pelikan & Yang Chai
We performed global gene expression analyses of the palatal and palatal tissue of Msx1-/- and control mice at embryonic day E14.5 Total RNA samples (1 ug per sample) were converted into biotin-labeled cDNA using the Enzo BioArray Terminal Labeling Kit with Biotin-ddUTP and standard protocols recommended by Affymetrix (Santa Clara, CA). Fragmented cDNA was applied to GeneChip, Mouse Genome 430 2.0 Arrays (Affymetrix) that contain probe sets designed to detect over 39,000 transcripts. Microarrays were...
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